Use of environmental sequencing in evaluating fungal response to peatland degradation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F24%3A43908501" target="_blank" >RIV/60076658:12310/24:43908501 - isvavai.cz</a>

Result on the web

<a href="http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/http://mires-and-peat.net/media/map31/map_31_17.pdf" target="_blank" >http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/http://mires-and-peat.net/media/map31/map_31_17.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.19189/MaP.2023.OMB.Sc.2319915" target="_blank" >10.19189/MaP.2023.OMB.Sc.2319915</a>

Result language

angličtina

Original language name

Use of environmental sequencing in evaluating fungal response to peatland degradation

Original language description

Central European peatlands are critically endangered by climate change, eutrophication, disturbed water regime and inappropriate management. This leads to their degradation, which affects all components of these habitats including fungi, and significantly fungi participating in the carbon cycle such as saprotrophs, mycorrhizal symbionts and parasites. Nature conservation efforts focus mainly on macrofungi, especially &apos;fungi important for nature conservation&apos; (FINC) which are rare or associated with endangered habitats. To find out whether standard environmental DNA (eDNA) sequencing methods can be used to monitor the effect of peatland degradation on FINC and fungal communities, we established pairs of plots in degraded and wellpreserved parts of ten peatlands in the T &amp; rcaron;ebo &amp; ncaron; Protected Landscape Area (Czech Republic). Peatland degradation was locally caused by a combination of different factors (water level and chemistry) followed by vegetation succession, whose effect on fungi was evaluated using multivariate analyses. A total of 28 FINC species were detected using macrofungal surveying, whereas 25 were found using eDNA of Sphagnum and peat (Illumina sequencing of ITS2). These two methods agreed on 13 predominantly abundant FINC species, most of which grew in both well-preserved and degraded plots. However, their sensitivity to the studied degradation could not be assessed due to the low abundance of most species. Fungal communities found on the basis of both methods (macrofungal survey, eDNA) were affected by water chemistry, whereas a significant effect of vegetation and locally expansive plants was demonstrated only in fungal communities found using macrofungal survey. Optimisation (e.g. specific primers) for local conditions leading to a better FINC capture must be carried out if eDNA is to be used in the practice of evaluating management effects on peatlands.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

<a href="/en/project/GA19-15031S" target="_blank" >GA19-15031S: Restoring biodiversity of disturbed peatlands as a basis for restoration of their future ecosystem functions and services</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mires and Peat

ISSN

1819-754X

e-ISSN

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Volume of the periodical

31

Issue of the periodical within the volume

2024

Country of publishing house

GB - UNITED KINGDOM

Number of pages

24

Pages from-to

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UT code for WoS article

001332999800001

EID of the result in the Scopus database

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