SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43910098" target="_blank" >RIV/60076658:12310/25:43910098 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70271?getft_integrator=clarivate&src=getftr&utm_source=clarivate" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70271?getft_integrator=clarivate&src=getftr&utm_source=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.70271" target="_blank" >10.1111/tpj.70271</a>
Alternative languages
Result language
angličtina
Original language name
SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses
Original language description
Cellular responses to abiotic stress involve multiple signals such as reactive oxygen species (ROS), Ca2+, abscisic acid (ABA), and chloroplast-to-nucleus retrograde signals such as 3 '-phosphoadenosine 5 '-phosphate (PAP). The mechanism(s) by which these messengers intersect for cell regulation remain enigmatic, as do the roles of retrograde signals in specialized cells. Here we demonstrate a mechanistic link enabling ABA and PAP to coordinate chloroplast and plasma membrane ROS production. Contrary to its role in upregulating processes leading to quenching of ROS in foliar tissue, we show that in guard cells, PAP induces chloroplast ROS accumulation via photosynthetic electron transport and apoplast ROS via the RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) proteins. Both subcellular ROS sources are necessary for stress hormone ABA-mediated stomatal closure, as well as PAP-mediated stomatal closure. However, PAP signaling diverges from ABA by activating RBOHD instead of RBOHF. Three calcium-dependent protein kinases (CPKs) transcriptionally induced by PAP, namely CPK13, CPK32, and CPK34, concurrently activate RBOHD and the slow anion channel SLAC1 by phosphorylating two SLAC1 serine (S) residues, including S120, which is also targeted by the ABA signaling kinase OPEN STOMATA 1 (OST1). Consequently, overexpression of the PAP-induced CPKs rescues stomatal closure in ost1. Our data identify chloroplast retrograde signals as critical nodes in cellular stress response networks of guard cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
1365-313X
Volume of the periodical
122
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
nestránkováno
UT code for WoS article
001508964000004
EID of the result in the Scopus database
2-s2.0-105008277891