IxsS7: A novel biomarker for Ixodes scapularis tick bite exposure in humans
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43911194" target="_blank" >RIV/60076658:12310/25:43911194 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1877959X25000780?getft_integrator=clarivate&pes=vor&utm_source=clarivate" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1877959X25000780?getft_integrator=clarivate&pes=vor&utm_source=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ttbdis.2025.102514" target="_blank" >10.1016/j.ttbdis.2025.102514</a>
Alternative languages
Result language
angličtina
Original language name
IxsS7: A novel biomarker for Ixodes scapularis tick bite exposure in humans
Original language description
Ixodes scapularis is a primary vector of several important tick-borne pathogens including Borrelia burgdorferi sensu lato, the causative bacterial genospecies complex of Lyme disease, Babesia microti, Anaplasma phagocytophilum, Borrelia miyamotoi, Ehrlichia muris eauclarensis, and Powassan virus. Salivary compounds secreted by I. scapularis during blood feeding are immunogenic and can elicit robust antibody responses in humans which can potentially be leveraged as surrogate markers of prior tick bite exposure. In this study, we investigate the potential of a tick secreted salivary serine protease inhibitor, IxsS7, as a novel antigenic biomarker of I. scapularis exposure in humans. We demonstrate that the IxsS7 protein-coding sequence is highly conserved (>90 % identity) among other important Ixodes species (e.g., Ixodes ricinus, Ixodes persulcatus, and Ixodes pacificus) and poorly conserved (<50 % identity) with homologs from other tick genera, such as Amblyomma spp., Dermacentor spp., Rhipicephalus spp., and Haemaphysalis spp. Antibodies in sera from rabbits immunized with recombinant IxsS7 (rIxsS7) strongly recognize native IxsS7 when challenged with salivary gland homogenate (SGH) from blood-fed I. scapularis females, while showing minimal cross-reactivity with SGH from other hard tick (Ixodidae) genera. Western blot and ELISA analyses revealed that human subjects who reported recent prior exposure to ticks possessed IgG antibodies that recognized rIxsS7, highlighting its potential as a biomarker of exposure specifically against I. scapularis. Further development of serological tools that can measure human antibody responses to Ixodesspecific salivary antigens is essential to better quantify individual- and population-level risk of important tickborne diseases such as Lyme disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Ticks and Tick-borne Diseases
ISSN
1877-959X
e-ISSN
1877-9603
Volume of the periodical
16
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
nestránkováno
UT code for WoS article
001536551800001
EID of the result in the Scopus database
2-s2.0-105010542201