The Role of Filippi's Glands in the Silk Moths Cocoon Construction
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12410%2F21%3A43903197" target="_blank" >RIV/60076658:12410/21:43903197 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/21:43903197 RIV/60077344:_____/21:00549900
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/24/13523/htm" target="_blank" >https://www.mdpi.com/1422-0067/22/24/13523/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms222413523" target="_blank" >10.3390/ijms222413523</a>
Alternative languages
Result language
angličtina
Original language name
The Role of Filippi's Glands in the Silk Moths Cocoon Construction
Original language description
Filippi's glands (FGs), formerly also called Lyonet's glands, are accessory secretory structures of the labial (silk) glands of lepidopteran caterpillars, which were implicated to play an important role in the maturation of the silk material and the construction of the cocoon. In our previous study, we have identified several species of giant silk moths that completely lack the FGs. Interestingly, the absence of FGs in these species correlates with the construction of a loose cocoon architecture. We investigated the functions of FGs by their surgical extirpation in the last instar larvae of the silkworm, Bombyx mori. We found that the absence of FGs altered the structure of the resulting cocoon, in which the different layers of silk were separated. In further experiments, we found no effects of the absence of FGs on larval cocoon formation behavior or on changes in cocoon mass or lipid content. Differential proteomic analysis revealed no significant contribution of structural proteins from FGs to silk cocoon material, but we identified several low abundance proteins that may play a role in posttranslational modifications of some silk proteins. Proteomic analysis also revealed a difference in phosphorylation of the N-terminal sequence of fibroin-heavy chain molecule. Thus, FGs appear to affect silk stickiness during spinning by regulating posttranslational modifications. This could also explain the link that exists between the absence of these glands and the formation of loose cocoons in some giant silk moth species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
24
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
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UT code for WoS article
000738542700001
EID of the result in the Scopus database
2-s2.0-85121146033