In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43872218" target="_blank" >RIV/60076658:12520/11:43872218 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2478/v10102-011-0014-x" target="_blank" >http://dx.doi.org/10.2478/v10102-011-0014-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/v10102-011-0014-x" target="_blank" >10.2478/v10102-011-0014-x</a>
Alternative languages
Result language
angličtina
Original language name
In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol
Original language description
Sexually mature pigs are known to possess high concentrations of testicular steroids, which have been shown to change the activities of cytochrome P450 in vitro. The aim of the present study was to evaluate the regulation of CYP1A and CYP2E1 activity bythe steroids dihydrotestosterone (DHT), 3beta-androstenol, 17beta-estradiol and 17alfa-estradiol. Catalytic activities of 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) were used as markers of CYP1A activities, while p-nitrophenol hydroxylace (PNPH) was used as a marker of CYP2E1 activities. Of the tested steroids, only 17beta-estradiol and 17alfa-estradiol inhibited EROD and MROD activities. This inhibition was observed when a steroid concentration of 100 mýM was used, while lower concentration showed no inhibitory effect. PNPH activities were inhibited only by 100 mýM of 17beta-estradiol. The significance of those results in vivo is unknown because inhibition was only found when concentrations of oes
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Interdisciplinary Toxicology
ISSN
1337-6853
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
7
Pages from-to
78-84
UT code for WoS article
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EID of the result in the Scopus database
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