Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883408" target="_blank" >RIV/60076658:12520/12:43883408 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aquaculture.2012.05.006" target="_blank" >http://dx.doi.org/10.1016/j.aquaculture.2012.05.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aquaculture.2012.05.006" target="_blank" >10.1016/j.aquaculture.2012.05.006</a>

Result language
angličtina
Original language name
Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping
Original language description
We describe spermatozoa characteristics from sequential collections in sterlet, Acipenser ruthenus, following a single dose of carp pituitary extract (CPE). Sperm production and spermatozoa fertilizing ability, percent motility, and curvilinear velocity(VCL) were investigated in fresh and frozen/thawed sperm. Sperm was collected by two procedures: (A) stripping 3 times per 24 h at 3 h intervals on 3 consecutive days beginning 12 h after CPE treatment, and (B) stripping 3 times over 6 h beginning 36 h after CPE treatment. Spermatozoa motility and VCL were evaluated by video microscopy, and sperm production was measured as volume and concentration. Sperm samples were frozen by a conventional freezing procedure in a cryoprotective medium containing 10% methanol. Fertilization was conducted using a ratio of 105 spermatozoa/egg. Both sequential stripping procedures yielded larger volumes of viable spermatozoa than did a single collection. Sperm parameters such as density and volume varied
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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