Comparison of three fluorescent CYP3A substrates in two vertebrate models: pig and Atlantic salmon
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883434" target="_blank" >RIV/60076658:12520/12:43883434 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1017/S1751731111002096" target="_blank" >http://dx.doi.org/10.1017/S1751731111002096</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1751731111002096" target="_blank" >10.1017/S1751731111002096</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of three fluorescent CYP3A substrates in two vertebrate models: pig and Atlantic salmon
Original language description
We investigated in vitro inhibitory effects of ketoconazole (KTZ) on cytochrome P450 activity in microsomes from pigs and Atlantic salmon. The following enzymatic reactions were studied: 7-benzyloxyresorufin and 7-ethoxyresorufin O-dealkylation (BROD andEROD, respectively), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD) and 7-benzyloxyquinoline O-debenzylation (BQOD). KTZ was a potent non-competitive inhibitor of BROD and BQOD in the microsomes from pigs, whereas in the microsomes from Atlantic salmon, these reactions were competitively inhibited by KTZ. BFCOD activity was inhibited by KTZ in a non-competitive manner in both species. KTZ non-competitively inhibited EROD in Atlantic salmon, but not in porcine microsomes. The activity ofBROD and BQOD was higher in male than that in female pigs, but the activity of BFCOD showed no sex-related differences.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal
ISSN
1751-7311
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
633-640
UT code for WoS article
000300714200014
EID of the result in the Scopus database
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