Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883467" target="_blank" >RIV/60076658:12520/12:43883467 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ecoenv.2011.12.012" target="_blank" >http://dx.doi.org/10.1016/j.ecoenv.2011.12.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ecoenv.2011.12.012" target="_blank" >10.1016/j.ecoenv.2011.12.012</a>
Alternative languages
Result language
angličtina
Original language name
Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure
Original language description
Little is known about the effects of the cardiovascular drug verapamil (VRP) on metabolic processes in fish. Most calcium channel blockers including VRP are metabolized by cytochrome P450 (CYP450) enzymes. In this study we investigated the in vivo effectof VRP on some CYP450-mediated reactions in juvenile rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of VRP (0.5, 27 and 270 mu g l(-1)) for 0, 21, and 42 day. The following CYP450-mediated reactions were studied in hepatic microsomes: O-dealkylation of ethoxyresorufin, methoxyresorufin, and pentoxyresorufin, hydroxylation of coumarin, tolbutamide, and p-nitrophenol, and O-debenzylation of 7-benzyloxy-4-trifluoromethylcoumarin. The amounts of products of these reactions did not differ among fish exposed to different levels of VRP and control fish. This suggests that the levels of VPR used did not alter catalytic activity of the selected CYP450 enzymes. In conclusion, none of the investigated CYP450-me
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN
0147-6513
e-ISSN
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Volume of the periodical
79
Issue of the periodical within the volume
05
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
148-152
UT code for WoS article
000302107000021
EID of the result in the Scopus database
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