Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886838" target="_blank" >RIV/60076658:12520/14:43886838 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs10695-014-9933-8" target="_blank" >http://link.springer.com/article/10.1007%2Fs10695-014-9933-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-014-9933-8" target="_blank" >10.1007/s10695-014-9933-8</a>

Result language

angličtina

Original language name

Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity

Original language description

In mammals, proteases are present in sperm acrosome and play key role in fertilization. Sturgeon sperm has an acrosome, but its physiology, biochemistry, and potential role in fertilization are unknown. In the present study, we have observed high protease activity in acidic extract of intact sperm compared to that of seminal plasma in sterlet (Acipenser ruthenus). The protease activity was decreased and increased in acidic extract of motility-activated sperm and in the activation medium, respectively. Molecular analysis revealed total protease and serine (acrosin) protease activities in sperm acidic extract which was accumulated in a protein band with relative molecular mass of 35 kDa. Immunoelectron microscopy using an affinity-purified polyclonal antibody for boar acrosin localized the protease at the acrosome region. Moreover, initiation of sperm motility was inhibited after activation in the presence of inhibitors for both trypsin-like and chymotrypsin-like proteases, while the eff

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

1393-1398

UT code for WoS article

000341498900008

EID of the result in the Scopus database

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