The Effect of Reactive Oxygen Species on Motility Parameters, DNA Integrity, Tyrosine Phosphorylation and Phosphatase Activity of Common Carp (Cyprinus carpio L.) Spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888471" target="_blank" >RIV/60076658:12520/15:43888471 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/mrd.22442/abstract;jsessionid=1CDC2A472DF296DAD4A24E74D6C47297.f02t01" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/mrd.22442/abstract;jsessionid=1CDC2A472DF296DAD4A24E74D6C47297.f02t01</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mrd.22442" target="_blank" >10.1002/mrd.22442</a>
Alternative languages
Result language
angličtina
Original language name
The Effect of Reactive Oxygen Species on Motility Parameters, DNA Integrity, Tyrosine Phosphorylation and Phosphatase Activity of Common Carp (Cyprinus carpio L.) Spermatozoa
Original language description
The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed todifferent concentrations of xanthine and xanthine oxidase (X-XO) either in the presence or absence of antioxidants for 15 and 60min. A dose- and time-dependent reduction in spermatozoa motility and velocity was observed. Comet assays showed a dramatic increase in DNA fragmentation after 15min. Changes in tyrosine phosphorylation of spermatozoa proteins were observed by Western blotting with anti-phosphotyrosine antibodies, and proteins of interest were identified by mass spectrometry. After a 60min exposure to X-XO, O-linked N-acetylglucosamine transferase, isoform 4 was phosphorylated and septin-8-A was dephosphorylated. Acid phosphatase activity also decreased in a dose-dependent manner after a 60min exposure to oxidative stress. The
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Reproduction and Development
ISSN
1040-452X
e-ISSN
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Volume of the periodical
82
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
48-57
UT code for WoS article
000348656700006
EID of the result in the Scopus database
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