Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888837" target="_blank" >RIV/60076658:12520/15:43888837 - isvavai.cz</a>
Result on the web
<a href="http://lipidworld.biomedcentral.com/articles/10.1186/s12944-015-0159-2" target="_blank" >http://lipidworld.biomedcentral.com/articles/10.1186/s12944-015-0159-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12944-015-0159-2" target="_blank" >10.1186/s12944-015-0159-2</a>

Result language
angličtina
Original language name
Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro
Original language description
Background: Human hepatocellular cells Hep G2 were used to mimic and investigate the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, fatty acid (FA) and phospholipid class composition. Methods: Cells were incubated with a combination of Cd2+ and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) fish specific FA. Results: We measured a significant increased proportion of EPA and DHA in the treated cells compared to the control line confirming the uptake. While doses of25 mu M DHA showed to be toxic to the cells, repeated short term incubations (2 h) at lower doses resulted in an increased uptake of DHA. The resarzurin assay, evaluating cell viability, showed a significant decrease in cell viability between Cd2+ incubation time and, further, the pre-incubation with BSA-FA complex resulted in significantly increased cell viability. On the metabolic level, increased concentrations of EPA and DHA resulted in an increased proportion of docosapentaenoic aci
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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