Technical note: Production of tetraploid sturgeons

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888852" target="_blank" >RIV/60076658:12520/15:43888852 - isvavai.cz</a>
Result on the web
<a href="https://dl.sciencesocieties.org/publications/jas/abstracts/93/8/3759" target="_blank" >https://dl.sciencesocieties.org/publications/jas/abstracts/93/8/3759</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2527/jas.2015-9094" target="_blank" >10.2527/jas.2015-9094</a>

Result language
angličtina
Original language name
Technical note: Production of tetraploid sturgeons
Original language description
Studies and practical application of androgenesis and gynogenesis in sturgeon are significantly hindered by strong influence of ploidy restoration treatment on survivability of progeny; therefore, developed method of production of tetraploid broodstock and, consequently, use of their diploid gametes might help to avoid ploidy restoration treatment. In the present study, for the first time was developed a protocol for tetraploidy induction in 2 model sturgeon species, sterlet (Acipenser ruthenus) and Siberian sturgeon (Acipenser baerii). A high efficiency of treatment was achieved by optimization of heat shock using a temperature of 37 degrees C for 2 min timed between the end of female pronuclei formation and the beginning of pronuclei migration, thatis, 0.8 to 1.0 tau(0) (duration of 1 mitotic cycle during the period of synchronous cleavage division). Fertilized eggs developed in tetraploid larvae, up to 31 (89.6% in control) and 34% (70.9% in control) in sterlet and Siberian sturgeo
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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