Identification of the light-independent phosphoserine pathway as an additional source of serine in the cyanobacterium Synechocystis sp PCC 6803

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888887" target="_blank" >RIV/60076658:12520/15:43888887 - isvavai.cz</a>

Result on the web

<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000055" target="_blank" >http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/mic.0.000055" target="_blank" >10.1099/mic.0.000055</a>

Result language

angličtina

Original language name

Identification of the light-independent phosphoserine pathway as an additional source of serine in the cyanobacterium Synechocystis sp PCC 6803

Original language description

L-Serine is one of the proteinogenic amino acids and participates in several essential processes in all organisms. In plants, the light-dependent photorespiratory and the light-independent phosphoserine pathways contribute to serine biosynthesis. In cyanobacteria, the light-dependent photorespiratory pathway for serine synthesis is well characterized, but the phosphoserine pathway has not been identified. Here, we investigated three candidate genes for enzymes of the phosphoserine pathway in Synechocystis sp. PCC 6803. Only the gene for the D-3-phosphoglycerate dehydrogenase is correctly annotated in the genome database, whereas the 3-phosphoserine transaminase and 3-phosphoserine phosphatase (PSP) proteins are incorrectly annotated and were identifiedhere. All enzymes were obtained as recombinant proteins and showed the activities necessary to catalyse the three-step phosphoserine pathway. The genes coding for the phosphoserine pathway were found in most cyanobacterial genomes listed

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbiology-SGM

ISSN

1350-0872

e-ISSN

—

Volume of the periodical

161

Issue of the periodical within the volume

Neuveden

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1050-1060

UT code for WoS article

000358186900012

EID of the result in the Scopus database

—