Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890383" target="_blank" >RIV/60076658:12520/16:43890383 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0166445X15300990" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0166445X15300990</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquatox.2015.11.017" target="_blank" >10.1016/j.aquatox.2015.11.017</a>

Result language

angličtina

Original language name

Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos

Original language description

Carbendazim is a widely used broad spectrum benzimidazole fungicide; however, its effects to non-target aquatic organisms are poorly studied. The aim of this study was to investigate the toxic effects of carbendazim to zebrafish early life stages at several levels of biological organization, including developmental, biochemical and behavioural levels. The embryo assay was done following the OECD guideline 236 and using a concentration range between 1.1 and 1.8 mg/L. Lethal and developmental endpoints such as hatching, edemas, malformations, heart beat rate, body growth and delays were assessed in a 96 h exposure. A sub-teratogenic range (from 0.16 to 500 mu g/L) was then used to assess effects at biochemical and behavioural levels. Biochemical markers included cholinesterase (ChE), glutathione-S-transferase (GST), lactate dehydrogenase (LDH) and catalase (CAT) and were assessed at 96 h. The locomotor behaviour was assessed using an automated video tracking system at 120 h. Carbendazim (96 h-LC50 of 1.75 mg/L) elicited several developmental anomalies in zebrafish embryos with EC50 values ranging from 0.85 to 1.6 mg/L. ChE, GST and LDH activities were increased at concentrations equal or above 4 mu g/L. The locomotor assay showed to be extremely sensitive, detecting effects in time that larvae spent swimming at concentrations of 0.16 a and thus, being several orders of magnitude more sensitive that developmental parameters or lethality. These are ecological relevant concentrations and highlight the potential of behavioural endpoints as early warning signs for environmental stress. Further studies should focus on understanding how the behavioural disturbances measured in these types of studies translate into fitness impairment at the adult stage.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

DN - Environmental impact on health

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquatic Toxicology

ISSN

0166-445X

e-ISSN

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Volume of the periodical

170

Issue of the periodical within the volume

Neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

390-399

UT code for WoS article

000368564500043

EID of the result in the Scopus database

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