Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890396" target="_blank" >RIV/60076658:12520/16:43890396 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0044848615301368" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848615301368</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2015.08.017" target="_blank" >10.1016/j.aquaculture.2015.08.017</a>

Result language

angličtina

Original language name

Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Original language description

To identify the effects of oocyte ageing on egg quality in northern pike, Esox lucius, two experiments were performed as follows: in experiment I, partial volumes of the eggs from 7 pike females were stripped and fertilised with 2-day intervals for 8 days post-ovulation (DPO). In experiment II, the stripped eggs of 3 females were stored in ovarian fluid at 10 degrees C and fertilised separately with 12-hour intervals for 96 hours post-stripping (HPS). In both experiments, the eggs from each batch were fertilised with mixed milt obtained from 5 males. Eyeing, hatching and eyed-egg mortality rates as well as the occurrence of larval malformations and ploidy anomalies were considered as indices for egg quality. The results of experiment I indicated that the best and most reliable postovulatory stripping times in pike were at 2 DPO and up to 4 DPO, respectively, when females were retained at 10 degrees C. Over-ripening of the eggs occurred from 8 days after ovulation. The eyed-egg mortality and larval malformation rates did not show any significant increase up to 6 DPO, but then increased significantly and reached 36% and 43% at 8 DPO, respectively. The incidence of triploid larvae increased over time, from 0% at 0 and 2 DPO to 11% and 14% in more aged oocytes at 4 and 6 DPO, respectively. The second experiment clarified that the best post-stripping fertilisation time in pike was at 0 HPS. However, stored eggs in ovarian fluid could retain almost 50% of their initial fertilising ability for 24 h after stripping. Complete loss of egg viability occurred after 96 HPS, and the highest eyed-egg mortality rate (27%) and larval malformation rate (33%) were detected in this group. The obtained results suggest that for commercial artificial reproduction procedures in northern pike, the time interval between successive fish examinations for ovulation should not exceed 4 days.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

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Volume of the periodical

450

Issue of the periodical within the volume

Neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

431-438

UT code for WoS article

000364854000058

EID of the result in the Scopus database

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