Alcalase treatment for elimination of stickiness in pikeperch (Sander lucioperca L.) eggs under controlled conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890536" target="_blank" >RIV/60076658:12520/16:43890536 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/are.12850/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/are.12850/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/are.12850" target="_blank" >10.1111/are.12850</a>

Result language

angličtina

Original language name

Alcalase treatment for elimination of stickiness in pikeperch (Sander lucioperca L.) eggs under controlled conditions

Original language description

Elimination of egg stickiness is an important factor in artificial reproduction of pikeperch (Sander lucioperca L.). This study was conducted to evaluate the effectiveness of Alcalase enzyme to remove the adhesive layer of pikeperch eggs. The eggs were treated with Alcalase at 0.5, 1.0, 1.5, 2.0 and 5.0 mL L-1 or a milk/talc solution 2 min post insemination. Duration of exposure was 2 min in Alcalase and 60 min in milk/talc. The highest, albeit not significant, hatching rate (85.4%) was found with 1.5 mL L-1 of Alcalase, but hatching rates were similar in 0.5, 1.0 and 2.0 mL L-1. Hatching rates were significantly lower groups treated with 5.0 mL L-1 Alcalase enzyme (56.4%) compared to groups treated with milk powder and talc (61.3%). Nominally complete removal of adhesiveness was observed in 1.5 and 2 mL L-1. All Alcalase treatments led to significantly lower incubation duration compared with the traditional milk/talc treatment. The application of Alcalase successfully eliminated pikeperch egg stickiness in less time than with traditional milk/clay/talc methods.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture Research

ISSN

1355-557X

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

3998-4003

UT code for WoS article

000387663000029

EID of the result in the Scopus database

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