The effect of GnRHa with or without dopamine inhibitor on reproductive hormone levels and sperm quality in tench Tinca tinca

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43892108" target="_blank" >RIV/60076658:12520/17:43892108 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0044848616305282" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848616305282</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2016.12.023" target="_blank" >10.1016/j.aquaculture.2016.12.023</a>

Result language

angličtina

Original language name

The effect of GnRHa with or without dopamine inhibitor on reproductive hormone levels and sperm quality in tench Tinca tinca

Original language description

The impact of treatment with mGnRHa, mGnRHa with metoclopramide, and metoclopramide on secretion of luteinizing hormone (LH), testosterone (T) and 11-ketotestosterone (11-KT) and on milt and spermatozoon characteristics was studied in male tench Tinca tinca. Male tench were divided into four groups and treated with following preparations: 0.9% NaCI; mGnRHa (25 mu g kg(-1)); mGnRHa (25 mu g kg(-1)) combined with metoclopramide (20 mg kg(-1)); and metoclopramide (20 mg kg(-1)). Administration of the combined treatment induced an LH surge at 12 h post injection (PI) reaching significantly higher values.(P &lt; 0.05) than was observed with stimulation by mGnRHa alone or with metoclopramide alone. Monitoring of LH levels demonstrated a rapid surge in LH release with the combined treatment versus gradual increase with mGnRHa alone (P &lt; 0.05). Experimental treatments with GnRHa alone, metoclopramide alone and metoclopramide combined with mGnRHa significantly increased milt production and relative number of spermatozoa kg(-1) BW at 24 h PI compared to controls, with no differences among experimental groups (P &lt; 0.05). No significant differences among groups (P &lt; 0.05) were found in motility or velocity (VLC, VAP, VSL). The only significant decrease of VLC and VAP was detected after treatment with metoclopramide alone compared to all other groups. Nonsignificant differences (P &lt; 0.05) were observed in T and 11-KT secretion, with the exception of a significant difference in T secretion (P &lt; 0.05) at 24 h PI between the combined group and the metoclopramide treatment and the controls. We conclude that the use of GnRH, combined or not with metoclopramide, are suitable hormonal treatments to control the spermiation in mature tench.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

—

Volume of the periodical

470

Issue of the periodical within the volume

1 March 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

91-94

UT code for WoS article

000394197900012

EID of the result in the Scopus database

2-s2.0-85007422224