Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43895334" target="_blank" >RIV/60076658:12520/17:43895334 - isvavai.cz</a>
Result on the web
<a href="http://www.publish.csiro.au/rd/RD16145" target="_blank" >http://www.publish.csiro.au/rd/RD16145</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1071/RD16145" target="_blank" >10.1071/RD16145</a>
Alternative languages
Result language
angličtina
Original language name
Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus
Original language description
The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20 degrees C and were designated 'TS after IVM' (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 208C) in the presence of 2 mMEGTA, 100 mu MVerapamil or 100 mu M Tetracaine. No motility was observed in the case of TS (10mM Tris, 25mM NaCl, 50mM Sucr with or without the addition of 2mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1-2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40103 - Fishery
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction, Fertility and Development
ISSN
1031-3613
e-ISSN
—
Volume of the periodical
29
Issue of the periodical within the volume
7
Country of publishing house
AU - AUSTRALIA
Number of pages
10
Pages from-to
1319-1328
UT code for WoS article
000403550200006
EID of the result in the Scopus database
2-s2.0-85021071705