mRNA abundance changes during invitro oocyte ageing in African catfish Clarias gariepinus (Burchell, 1822)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43896168" target="_blank" >RIV/60076658:12520/18:43896168 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/are.13552/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/are.13552/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/are.13552" target="_blank" >10.1111/are.13552</a>

Result language

angličtina

Original language name

mRNA abundance changes during invitro oocyte ageing in African catfish Clarias gariepinus (Burchell, 1822)

Original language description

Delayed fertilization following ovulation leads to the oocyte ageing which has been identified as the most important factor affecting egg quality after ovulation. Very little is known about the molecular changes associated with the progress of oocyte ageing. The present study monitored the egg viability rates during post-stripping oocyte ageing in African catfish Clarias gariepinus. In addition the mRNA abundance of selected genes were studied during the progress of oocyte ageing by real time quantitative PCR. To study how maternal transcripts influence egg quality, expression levels were correlated with egg hatching rates. The highest embryo survival and hatching rates (88% and 81%, respectively) were obtained from eggs that were fertilized immediately after stripping. Complete loss of egg viability occurred at 16 and 24 hr Post Stripping (HPS) when eggs were stored at 25 degrees C and 4 degrees C respectively. Under both storage temperatures, the embryo mortality and larval malformation rates increased significantly over time and were the highest in the most aged oocytes. Genes indicating an upward trend in expression during ova ageing were determined to be related to oxidative injury and the stress response (hsp70), mitochondrial function (calmodulin), apoptosis (cathepsin D) and germ line speciation (vasa and sox9a). The results suggest that oxidative stress and mitochondrial dysfunction might be associated with post-stripping ova ageing and the consequent induction of egg quality defects. The examined genes may be considered as candidate markers of egg quality associated with oocyte ageing in African catfish.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture Research

ISSN

1355-557X

e-ISSN

—

Volume of the periodical

49

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1037-1045

UT code for WoS article

000419478000049

EID of the result in the Scopus database

2-s2.0-85041460848