Development of a robust extraction procedure for the HPLC-ESI-HRPS determination of multi-residual pharmaceuticals in biota samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43897129" target="_blank" >RIV/60076658:12520/18:43897129 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0003267018304872" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267018304872</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2018.04.011" target="_blank" >10.1016/j.aca.2018.04.011</a>
Alternative languages
Result language
angličtina
Original language name
Development of a robust extraction procedure for the HPLC-ESI-HRPS determination of multi-residual pharmaceuticals in biota samples
Original language description
A simple, robust and effective extraction procedure for the determination of 74 pharmaceuticals in different fish tissues by ultrasensitive high performance liquid chromatography with electrospray high resolution product scan (HPLC-ESI-HRPS) was developed and validated. Different extraction solvent mixtures were tested to achieve the highest recoveries of the selected analytes, to minimize the influence of a complex matrix and to reduce the total analysis time as well as cost of analysis. A mixture of acetonitrile + isopropanol (3:1 v/v) acidified with 0.1% formic acid was the best extraction solvent among the five solvents tested for most of the tissues with the exception of plasma samples, where only acidified acetonitrile exhibited the best performance. The developed method was validated at three concentration levels (5, 20 and 50 ng g-1) in five different fish tissues (liver, kidney, brain, muscle and plasma). Most of the target analytes were extracted with a recovery between 60 and 130%. Very low limits of quantification (LOQs) were obtained for the majority of the pharmaceuticals in all of the studied matrices. The developed analytical method was successfully applied for the analysis of common carp (Cyprinus carpio) originating from the waste water effluent-dominated pond Cezarka (Czech Republic). The results confirmed the importance of multi-tissue analysis to obtain complex information on the distribution of pharmaceuticals in fish.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
1022
Issue of the periodical within the volume
8/2018
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
53-60
UT code for WoS article
000432333600006
EID of the result in the Scopus database
2-s2.0-85046144842