D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43898152" target="_blank" >RIV/60076658:12520/18:43898152 - isvavai.cz</a>

Result on the web

<a href="https://www.cambridge.org/core/journals/animal/article/d1-but-not-d2-dopamine-receptor-regulates-steroid-levels-during-the-final-stages-of-pikeperch-gametogenesis/63DF51686C65A5368E2E5AC07758A848" target="_blank" >https://www.cambridge.org/core/journals/animal/article/d1-but-not-d2-dopamine-receptor-regulates-steroid-levels-during-the-final-stages-of-pikeperch-gametogenesis/63DF51686C65A5368E2E5AC07758A848</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1751731118000824" target="_blank" >10.1017/S1751731118000824</a>

Result language

angličtina

Original language name

D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Original language description

In pikeperch, Sander lucioperca, aquaculture hormonal treatment is usually applied to synchronize ovulation. However, the effect of dopamine (DA) receptor antagonists, in particular those blocking the D1 DA receptors, remains unknown. Thus, the aim of the present study was to investigate and compare the effects of D1 and D2 DA receptor antagonists on the sex-steroid production and reproductive performance of the species. Two experiments were performed during which mature pikeperch females were injected with different molecules: NaCl 0.9% (negative control) or human chorionic gonadotropin 500 IU/kg (positive control) in both experiments, metoclopramide (a D2 receptor antagonist 4 mg/kg or 20 mg/kg) or SCH23390 (a D1 receptor antagonist 0.8 mg/kg or 4 mg/kg) alone (experiment 1) or in combination with a salmon gonadotropin-releasing hormone analogue (sGnRHa at 25 mu g/kg; experiment 2). In experiment 2, fish were also injected with sGnRHa (25 mu g/kg) as positive control. Samplings of oocytes and blood were performed on the day of injection and after 24 h (both experiments), after 48 h (experiment 2) and at the time of ovulation (both experiments). In non-ovulating fish, samplings were performed 7 days (experiment 1) or 14 days (experiment 2) after injection. In experiment 2, various zootechnical parameters of fertilized eggs were recorded (survival, hatching and malformation rates). The two antagonists alone were ineffective in inducing the final stages and regulating sex-steroid (testosterone, 11 ketotestosterone, 17 beta estradiol and 17,20 beta-dihydroxy-4-pregnen-3-one) production. When administered with sGnRHa, both SCH23390 and metoclopramide induced the final stages. However, only SCH23390 stimulated testosterone (4 mg/kg) and 17 beta estradiol (0.8 mg/kg) production compared with sGnRHa alone. None of the treatments affected the survival, hatching or malformation rates. This is the first report suggesting that in pikeperch the D1, but not the D2, DA receptor antagonist would be involved in the testosterone and 17 beta estradiol production as a potentiator of the sGnRHa effect.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal

ISSN

1751-7311

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

2587-2597

UT code for WoS article

000450622800017

EID of the result in the Scopus database

2-s2.0-85045732741