Antioxidant defence system in protein fractions of common carp (Cyprinus carpio) seminal plasma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899218" target="_blank" >RIV/60076658:12520/19:43899218 - isvavai.cz</a>

Result on the web

<a href="https://www.agriculturejournals.cz/publicFiles/187_2018-CJAS.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/187_2018-CJAS.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/187/2018-CJAS" target="_blank" >10.17221/187/2018-CJAS</a>

Result language

angličtina

Original language name

Antioxidant defence system in protein fractions of common carp (Cyprinus carpio) seminal plasma

Original language description

There are growing evidences that the damage which is caused to the spermatozoa by the reactive oxygen species (ROS) plays a key role in male infertility. The seminal plasma is endowed with many enzymatic and nonenzymatic antioxidants which protect the spermatozoa against oxidative stress. The present study was undertaken to compare the level of superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx) in separated protein fractions of whole common carp Cyprinus carpio seminal plasma. Seminal plasma from six male common carp was separated into four fractions. The fractions differed from each other in their total seminal plasma protein content and antioxidant status. The highest value of SOD (2.81 +/- 0.11 mU/mg protein) was found in fraction 4, and the lowest (1.83 +/- 0.18 mU/mg protein) in fraction 1. There were significant variations (ANOVA; P = 0.05) in GR activity, mainly between fractions 1, 2, and 4. The lowest value of GPx activity (14.9 +/- 0.4 mU/mg protein) was found in fraction 2; however, the GPx activity showed low variability among fractions. We conclude that distinct seminal plasma fractions differ in antioxidant capacity. The inclusion of fractions with high levels of SOD, GR, and GPx in sperm storage medium may enhance protection against oxidative stress and promote sperm survival during the in vitro storage.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science : Živočišná výroba

ISSN

1212-1819

e-ISSN

—

Volume of the periodical

64

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

265-271

UT code for WoS article

000471878000004

EID of the result in the Scopus database

2-s2.0-85068642111