Cryopreservation of Germ Stem Cells in Fish
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901834" target="_blank" >RIV/60076658:12520/20:43901834 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cryopreservation of Germ Stem Cells in Fish
Original language description
Germ cells have drawn much attention as gamete precursors because of their exceptional potential for fundamental biological studies, as well as for being a potential alternative to gene resource banking for breeding and restocking purposes via surrogacy. Research has developed markers for germ cell identification, shedding light on their origin and specification mechanism. Then, pioneering studies focused on germ cell transplantation into the surrogate host with donor-derived gamete production have followed suit. The advent of transgenic technologies allowed the development of lines expressing green fluorescent protein (GFP) in their germ cells exclusively or the use of artificially synthesised mRNA conjugated with GFP to label and trace germ cells in vivo and facilitate studies on transplantation. The power of germ cell manipulation techniques was significantly enhanced by the development of a wide range of cryopreservation methods; with these approaches, valuable genetic material can be cryopreserved even during the blastula stage, as well as harvested from an adult specimen. In turn, the germ cells in the cryogenic storage can be recovered by transplantation in the surrogate host, producing donor-derived gametes after maturation. The aim of this chapter is to provide basic knowledge about a relatively novel biotechnological approach in fish reproduction and summarise performed studies on germ cell cryopreservation.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Cryopreservation of Fish Gametes
ISBN
978-981-15-4024-0
Number of pages of the result
28
Pages from-to
285-312
Number of pages of the book
352
Publisher name
Springer Nature Singapore Pte Ltd.
Place of publication
Singapore
UT code for WoS chapter
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