Specificity of Germ Cell Technologies in Sturgeons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901835" target="_blank" >RIV/60076658:12520/20:43901835 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Specificity of Germ Cell Technologies in Sturgeons

Original language description

Sturgeons are one of the oldest, biggest, most valuable and today also most endangered group of fish species. Germ stem cells (GSCs), such us embryonic primordial germ cells (PGCs) or spermatogonial/oogonial stem cells, can be a key for an effective conservation and possible restoration of these unique and astonishing fishes. In this chapter, labeling, development, isolation, and transplantation of GSCs were studied in sturgeons. It was shown that the maternally supplied germ plasm, which determines the PGC origin, is localized in vegetal pole of ovulated egg and remains there throughout the cleavage period, and therefore, the PGC specification pattern is similar to that of anuran amphibians rather than teleostean fishes. This knowledge enabled to develop an original PGC labeling method using common cell tracer dye injection into the vegetal pole of two- to eight-cell stage embryo. Next inhibition of maternally supplied dead end RNA resulted in PGC mismigration and general sterilization of individuals. This method enables preparation of recipients for germ cell transplantation. Isolation and transplantation of spermatogonia and oogonia were developed as well. It was tested that one sturgeon juvenile (Siberian sturgeon) can provide approximately one million germ cells suitable for transplantation. Moreover, it was shown that these cells are capable of propagation via an in vitro culture system and of cryopreservation. After freezing/thawing of sturgeon gonadal tissue followed by enzymatic dissociation, above 90% of viable cells were obtained and used for transplantation. The technique of surrogate production can be applied for conservation and possibly restoration of critically endangered sturgeon species with a long term of maturation and a big body size (e.g., beluga), whereas a more common species with shorter term of maturation and smaller body size (e.g., sterlet) can be used as a recipient (surrogate parent).

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Reproduction in Aquatic Animals

ISBN

978-981-15-2289-5

Number of pages of the result

22

Pages from-to

335-356

Number of pages of the book

379

Publisher name

Springer Nature Singapore Pte Ltd.

Place of publication

Singapore

UT code for WoS chapter

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