Multiple sperm collection as an effective solution for gamete management in pikeperch (Sander lucioperca)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902485" target="_blank" >RIV/60076658:12520/21:43902485 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2020.735870" target="_blank" >https://doi.org/10.1016/j.aquaculture.2020.735870</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2020.735870" target="_blank" >10.1016/j.aquaculture.2020.735870</a>

Result language

angličtina

Original language name

Multiple sperm collection as an effective solution for gamete management in pikeperch (Sander lucioperca)

Original language description

The aims of this study were to investigate (a) the effects of single or multiple sperm collection with catheter, and (b) impacts of multiple sampling method (catheter or syringe) on sperm quality in pikeperch (Sander lucioperca). To compare the effects of sampling method, sperm was collected repeatedly from the same fish using catheter (n = 6) or syringe (n = 5) at 48, 52 and 56 h post-hormonal injection. For the single collection group as control, sperm was collected with catheter from 12 fish at 48 h post-injection. The results showed that multiple sperm collection with catheter does not significantly (P&gt; 0.05) influence sperm volume and density compared to single sperm collection. However, multiple collection with syringe led to drastic reduction of sperm density at 56 h post-injection. Moreover, sperm volume was influenced by post-injection time where the highest volume was obtained at 48 h post-injection. Furthermore, sperm density and osmolality of seminal plasma were significantly affected by sampling method and post-injection time where higher values were found for the samples collected with catheter at 48 h post-injection. Number of males producing milt with motile spermatozoa decreased along with multiple sperm collection. Sperm motility at 60 s post-activation was affected by the interaction of sampling method and post-injection time, and the highest value was obtained for the sperm collected with syringe at 48 h post-injection. Sperm curvilinear velocity (VCL) at 15 and 45 s post-activation was affected by sampling method and post-injection time, respectively. These findings contribute to the pikeperch hatchery practices, as multiple sperm collection could be applied during artificial reproduction if limited number of males are available.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

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Volume of the periodical

530

Issue of the periodical within the volume

neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

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UT code for WoS article

000582169700020

EID of the result in the Scopus database

2-s2.0-85089946527