Egg-sperm interaction in sturgeon: role of ovarian fluid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902546" target="_blank" >RIV/60076658:12520/21:43902546 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s10695-020-00852-2" target="_blank" >https://doi.org/10.1007/s10695-020-00852-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-020-00852-2" target="_blank" >10.1007/s10695-020-00852-2</a>

Result language

angličtina

Original language name

Egg-sperm interaction in sturgeon: role of ovarian fluid

Original language description

Fertilization of freshwater fish occurs in the environment which negatively affects a lifespan of gametes mostly due to the osmotic shock; therefore, male gametes should reach the female gamete, as soon as possible. The existence of mechanisms controlling the encounter of gametes would be highly expedient in this case. By analogy with other species for which guidance was demonstrated, it is likely that this control may be performed by ovarian fluid or substances released by eggs. The aim was to study the effect of ovarian fluid and egg-released substances on spermatozoa behavior in sterlet. It was found that the presence of a particular concentration of ovarian fluid (30% solution in water) had an inhibiting effect on spermatozoa motility initiation. Lower concentrations of the ovarian fluid improved the longevity of spermatozoa and did not affect their trajectories. Test of chemotactic response (using a microcapillary injection of fluids into the suspension of motile spermatozoa) showed no effect of ovarian fluid on spermatozoa behavior, while at the same time, the attracting effect of the egg-conditioned medium was evident (i.e., due to some substances released from the eggs during their contact with freshwater). The results of the fertilization test showed that the presence of ovarian fluid prevented the eggs from losing the fertilizing ability due to the contact with water, as well as promoted the spermatozoa to fertilize the eggs during a longer period of time. Thus, the combined physicochemical action of &quot;female factors&quot; affects sterlet gametes during fertilization and may be involved in the guidance and selection mechanisms.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

—

Volume of the periodical

47

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

17

Pages from-to

653-669

UT code for WoS article

000560612300001

EID of the result in the Scopus database

2-s2.0-85089444747