Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902708" target="_blank" >RIV/60076658:12520/21:43902708 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/21:10436149

Result on the web

<a href="https://doi.org/10.3390/photonics8080333" target="_blank" >https://doi.org/10.3390/photonics8080333</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/photonics8080333" target="_blank" >10.3390/photonics8080333</a>

Result language

angličtina

Original language name

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Original language description

The most realistic information about a transparent sample such as a live cell can be obtained using bright-field light microscopy. Under high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped with a high-resolution (4872 x 3248) image sensor. In order to suppress data distortions originating from the light interactions with elements in the optical path, poor sensor reproduction (geometrical defects of the camera sensor and some peculiarities of sensor sensitivity), we propose a spectroscopic approach for the correction of these uncompressed 12 bpc data by simultaneous calibration of all parts of the experimental arrangement. Moreover, the final intensities of the corrected images are proportional to the photon fluxes detected by a camera sensor. It can be visualized in 8 bpc intensity depth after the Least Information Loss compression.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

<a href="/en/project/LM2018099" target="_blank" >LM2018099: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photonics

ISSN

2304-6732

e-ISSN

2304-6732

Volume of the periodical

8

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

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UT code for WoS article

000690188000001

EID of the result in the Scopus database

2-s2.0-85113770395