Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902836" target="_blank" >RIV/60076658:12520/21:43902836 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2021.737113" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.737113</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2021.737113" target="_blank" >10.1016/j.aquaculture.2021.737113</a>

Result language

angličtina

Original language name

Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

Original language description

This study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 +/- 0.21 g) were fed eight experimental diets containing two lipid levels of 9% (basal-fat diet, BFD) and 18% (high-fat diet, HFD), and four levels of KGM (0, 0.5, 1 and 2%) for 60 days. Pompano fed HFD exhibited markedly lower growth and feed efficiency than fish fed BFD, and KGM supplementation, particularly at 1% inclusion, significantly improved growth performance and feed efficiency in fish fed both BFD and HFD. In addition, Pompano fed HFD showed higher whole-body lipid and lower protein contents than fish fed BFD. Irrespective of dietary lipid level, whole body, dorsal and ventral muscle were reduced by dietary KGM supplementation up to 1% while the opposite was found for whole body protein content. Dietary KGM also significantly reduced the HSI, liver lipid contents, as well as lipid droplets in the liver. Supplementation of KGM to both BFD and HFD significantly reduced serum total cholesterol, triglyceride, lowdensity lipoprotein cholesterol and free fatty acids levels. Furthermore, irrespective of dietary lipid level, increasing dietary KGM supplementation enhanced serum IgM level, lysozyme and alkaline phosphatase activities, and reduced serum alanine aminotransferase and aspartate aminotransferase activities. Dietary KGM also increased liver total antioxidant capacity, superoxide dismutase and catalase activities, and decreased malondialdehyde concentration in fish fed both HFD and BFD. Dietary KGM significantly increased serum glutathione peroxidase, superoxide dismutase and catalase activities and reduced malondialdehyde concentrations in fish fed BFD, and enhanced serum total antioxidant capacity and glutathione peroxidase activity in fish fed HFD. Supplementation of KGM increased the expression of genes associated with lipid catabolism (ppar alpha, cpt1, hsl) and lipid transport (fabp1, apoB100 and cd36), and decreased the expression of lipid anabolism related genes (srebp1, ppar gamma, fas and acc). Moreover, the expression of pro-inflammatory cytokines including tumor necrosis factor alpha and interleukin-8 were down-regulated, while expression of the anti-inflammatory cytokine transforming growth factor beta 1 was increased by KGM supplementation. In conclusion, supplementation of KGM at 1% of diet improved growth, feed efficiency, antioxidant capacity, hepatic lipid metabolism and inflammatory responses in juvenile pompano, and can mitigate excessive deposition of lipid in tissues of farmed fish.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

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Volume of the periodical

545

Issue of the periodical within the volume

neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

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UT code for WoS article

000692122600009

EID of the result in the Scopus database

2-s2.0-85111614255