An Improved Transwell Design for Microelectrode Ion-Flux Measurements

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3AN0000002" target="_blank" >RIV/60076658:12520/21:N0000002 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/21:00543177

Result on the web

<a href="https://www.mdpi.com/2072-666X/12/3/273/htm" target="_blank" >https://www.mdpi.com/2072-666X/12/3/273/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/mi12030273" target="_blank" >10.3390/mi12030273</a>

Result language

angličtina

Original language name

An Improved Transwell Design for Microelectrode Ion-Flux Measurements

Original language description

The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. We introduce a measurement window in the transwell insert membrane, which provides direct access for the cells to the media in the upper and lower compartment of the transwell system and allows direct cell-to-cell contact coculture. Three-dimensional multiphoton lithography (MPL) was used to construct a 3D grid structure for cell support in the measurement window. The optimal polymer grid constant was found for implementation in transwell MIFE measurements. We showed that human umbilical vein endothelial cells (HUVECs) efficiently grow and maintain their physiological response on top of the polymer structures.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

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Continuities

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Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MICROMACHINES

ISSN

2072-666X

e-ISSN

2072-666X

Volume of the periodical

13

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

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UT code for WoS article

000633929700001

EID of the result in the Scopus database

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