Partial fads2 Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Delta 8 Pathway with an Impact on the Reproduction of Female Zebrafish (Danio rerio)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904466" target="_blank" >RIV/60076658:12520/22:43904466 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3390/genes13040700" target="_blank" >https://doi.org/10.3390/genes13040700</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/genes13040700" target="_blank" >10.3390/genes13040700</a>

Result language

angličtina

Original language name

Partial fads2 Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Delta 8 Pathway with an Impact on the Reproduction of Female Zebrafish (Danio rerio)

Original language description

The zebrafish (Danio rerio) genome contains a single gene fads2 encoding a desaturase (FADS2) with both Delta 6 and Delta 5 activities, the key player in the endogenous biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs), which serve essential functions as membrane components, sources of energy and signaling molecules. LC-PUFAs include the precursors of eicosanoids and are thus predicted to be indispensable molecules for reproductive health in virtually all vertebrates. In mice, an amniotic vertebrate, fads2 deletion mutants, both males and females, have been confirmed to be sterile. In anamniotic vertebrates, such as fish, there is still no information available on the reproductive (in)ability of fads2 mutants, although zebrafish have become an increasingly important model of lipid metabolism, including some aspects of the generation of germ cells and early embryonic development. In the present study, we apply the CRISPR/Cas9 genome editing system to induce mutations in the zebrafish genome and create crispants displaying a degree of fads2 gene editing within the range of 50-80%. Focusing on adult G(0) crispant females, we investigated the LC-PUFA profiles of eggs. Our data suggest an impaired pathway of the LC-PUFA biosynthesis of the omega 6 and omega 3 series in the first-rate limiting steps of the conversion of linoleic acid (LA) into gamma-linolenic acid (GLA), and alpha-linolenic acid (ALA) into stearidonic acid (SDA), respectively, finally resulting in bad-quality eggs. Our data suggest the existence of an alternative Delta 8 pathway, which bypasses the first endogenous LC-PUFA biosynthetic step in zebrafish in vivo, and suggest that the zebrafish bifunctional FADS2 enzyme is actually a trifunctional Delta 6/Delta 5/Delta 8 desaturase.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genes

ISSN

2073-4425

e-ISSN

2073-4425

Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

nestrankovano

UT code for WoS article

000785468900001

EID of the result in the Scopus database

2-s2.0-85125652749