Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904535" target="_blank" >RIV/60076658:12520/22:43904535 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.aqrep.2022.101160" target="_blank" >https://doi.org/10.1016/j.aqrep.2022.101160</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2022.101160" target="_blank" >10.1016/j.aqrep.2022.101160</a>

Result language

angličtina

Original language name

Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery

Original language description

The purpose of this study was to investigate the effect of an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec solution (5 mM KCl, 45 mM NaCl, 30 mM Tris-HCl; pH 8.0 and 130 mOsm/kg) and Cheng solution (40 mM NaCl, 20 mM Tris; pH 8.0 and 100 mOsm/kg) on aging sperm of common carp (Cyprinus carpio) after storage in a carp artificial seminal plasma (carp ASP: 2 mM CaCl2, 1 mM MgSO4, 40 mM KCl, 110 mM NaCl, 20 mM Tris; pH 7.5 and 310 mOsm/kg) for 7 days without antibiotics at 4 degrees C. Fresh milt and undiluted aging milt were used as the control groups. Milt (5 ml) was collected and diluted 1:1 in a carp ASP in 50 ml containers and stored at 4 degrees C under aerobic conditions. The highest sperm motility was found 45% and 36% at 15 s and 45 s post sperm activation (PSA) in hatchery water. Additionally, the curvilinear velocity (VCL, mu m/s) and straight-line velocity (VSL, mu m/s) of sperm were the highest in hatchery water and the solution with 5 mM KCl, 45 mM NaCl, 30 mM Tris and pH 8.0, which were above 122 mu m/s and 87 mu m/s at 15 PSA. Sperm concentration in aging sperm of common carp is known to decrease rapidly, so prior to fertilization, sperm concentration was determined and accurately set to 200,000 sperm per egg. The fertilization of eggs and hatching rate after 7 days of sperm storage in carp ASP were 85-87% and 78-81% in both ASs of hatchery water and Perchec solution. Less than 1% sperm motility, fertilization and hatching rate were observed in undiluted sperm (control). In conclusion, the fertilization ability of aging common carp sperm in carp ASP within different ASs was confirmed for practical use in aquaculture.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

nestrankovano

UT code for WoS article

000799948200004

EID of the result in the Scopus database

2-s2.0-85129935516