Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904604" target="_blank" >RIV/60076658:12520/22:43904604 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3390/fishes7050248" target="_blank" >https://doi.org/10.3390/fishes7050248</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/fishes7050248" target="_blank" >10.3390/fishes7050248</a>

Result language

angličtina

Original language name

Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna

Original language description

The influence of water hardness on copper (Cu) toxicity in Daphnia magna was studied using gene expression analysis. Exposing D. magna to Cu in water with increasing levels of hardness decreased the acute toxicity. Hardness did not affect the predicted Cu complexation. After 24 h, D. magna showed an increased level of genes related to metal homeostasis (mt) following exposure to 25 mu g Cu/L in hard water. Daphnids in soft and medium water responded to 25 mu g Cu/L by upregulation of antioxidant defense and mt genes, revealing oxidative stress as a mechanism of Cu toxicity in D. magna. D. magna exposed to 25 mu g Cu/L in soft water did not survive for 96 h. In contrast, those exposed to 25 mu g Cu/L in medium and hard water survived for 96 h with significantly higher levels of mt genes. The genes related to oxidative damage (heat shock protein and glutathione S-transferase) in these groups did not deviate from control levels, indicating the protective effect of hardness. Metallothionein genes were upregulated at 17 mu g Cu/L at both 24 h and 96 h. The expression of catalase and ferritin increased in this group in soft and hard water at 96 h. The protective effect of hardness (in the tested range) on survival was also observed at a concentration of 25 mu g/L. The results suggest metallothionein (A and B), catalase, and ferritin genes, as potential biomarkers for copper exposure in D. magna regardless of hardness.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10613 - Zoology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fishes

ISSN

2410-3888

e-ISSN

2410-3888

Volume of the periodical

7

Issue of the periodical within the volume

5

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

nestrankovano

UT code for WoS article

000872724100001

EID of the result in the Scopus database

2-s2.0-85140629123