Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904618" target="_blank" >RIV/60076658:12520/22:43904618 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3389/fvets.2022.1036495" target="_blank" >https://doi.org/10.3389/fvets.2022.1036495</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2022.1036495" target="_blank" >10.3389/fvets.2022.1036495</a>

Result language

angličtina

Original language name

Optimization of in vitro culture conditions of common carp germ cells for purpose of surrogate production

Original language description

Common carp (Cyprinus carpio) is the fourth most-produced fish species in aquaculture and frequently used model species with significant effort invested in development of biotechnological applications. In present study, we attempted to establish an in vitro germ cell culture condition for short term cell culture, which could facilitate further applications such as surrogacy or gene manipulation. Basal media and different types of feeder cells were investigated to optimize carp germ cell culture condition to favor maintenance of mitotic proliferation. Results indicated that germ cells cultured with hESC media and RTG2 cell line as feeder possessed significantly higher proliferation and survival rate compared to that cultured with StemPro media and Sertoli cell line as feeder. In addition, we compared two dissection strategies to compare risk of cell culture contamination and body cavity was open from dorsal part or from ventral part. As a result, carp open from the dorsal side can minimize the risk of contamination. In summary, this is the first study to optimize the cultivation of germ cells in common carp. This opens up new opportunities for the application of specific techniques in the breeding of those species with high commercial value and frequent use as a model fish. Results obtained in this study are important for implementation of new strategies in common carp breeding, conservation of genetic resources, restoration of lines or development of clonal and isogenic carp lines.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Veterinary Science

ISSN

2297-1769

e-ISSN

2297-1769

Volume of the periodical

9

Issue of the periodical within the volume

neuvedeno

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

nestrankovano

UT code for WoS article

000876987500001

EID of the result in the Scopus database

2-s2.0-85141092596