Evaluation of absorbent cotton for glycopeptide enrichment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43905241" target="_blank" >RIV/60076658:12520/22:43905241 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00216-022-04353-4" target="_blank" >https://doi.org/10.1007/s00216-022-04353-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-022-04353-4" target="_blank" >10.1007/s00216-022-04353-4</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of absorbent cotton for glycopeptide enrichment
Original language description
Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples. Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer. The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans. Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200 mu L tip achieved a reasonable glycopeptide enrichment performance (similar to 6% recovery) from similar to 0.5mg peptides. In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
1618-2650
Volume of the periodical
414
Issue of the periodical within the volume
29-30
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
8245-8253
UT code for WoS article
000862528800004
EID of the result in the Scopus database
2-s2.0-85139235783