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Intracytoplasmic sperm injection in sturgeon species: A promising reproductive technology of selected genitors

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43905249" target="_blank" >RIV/60076658:12520/22:43905249 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.3389/fvets.2022.1054345" target="_blank" >https://doi.org/10.3389/fvets.2022.1054345</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fvets.2022.1054345" target="_blank" >10.3389/fvets.2022.1054345</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Intracytoplasmic sperm injection in sturgeon species: A promising reproductive technology of selected genitors

  • Original language description

    Sturgeons are the most endangered species group and their wild populations continue to decrease. In this study, we apply intracytoplasmic sperm injection (ICSI), an assisted reproductive technology, for the first time in endangered and critically endangered sturgeons. Using various egg-sperm species combinations we performed different ICSI experiments with immobilized pre- or non-activated spermatozoa, single or many, fresh or cryopreserved. Then we evaluated the fertilization success as well as the paternity of the resultant embryos and larvae. Surprisingly, all experimental groups exhibited embryonic development. Normal-shaped feeding larvae produced in all egg-sperm species-combination groups after ICSI using single fresh-stripped non-activated spermatozoa, in one group after ICSI using single fresh-stripped pre-activated spermatozoa, and in one group after ICSI using multiple fresh-stripped spermatozoa. ICSI with single cryopreserved non-activated spermatozoa produced neurula stage embryos. Molecular analysis showed genome integration of both egg- and sperm-donor species in most of the ICSI transplants. Overall, ICSI technology could be used as an assisted reproduction technique for producing sturgeons to rescue valuable paternal genomes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10604 - Reproductive biology (medical aspects to be 3)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Veterinary Science

  • ISSN

    2297-1769

  • e-ISSN

    2297-1769

  • Volume of the periodical

    9

  • Issue of the periodical within the volume

    neuvedeno

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    14

  • Pages from-to

    nestrankovano

  • UT code for WoS article

    000907793800001

  • EID of the result in the Scopus database

    2-s2.0-85145654866