Intracytoplasmic sperm injection in sturgeon species: A promising reproductive technology of selected genitors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43905249" target="_blank" >RIV/60076658:12520/22:43905249 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3389/fvets.2022.1054345" target="_blank" >https://doi.org/10.3389/fvets.2022.1054345</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fvets.2022.1054345" target="_blank" >10.3389/fvets.2022.1054345</a>
Alternative languages
Result language
angličtina
Original language name
Intracytoplasmic sperm injection in sturgeon species: A promising reproductive technology of selected genitors
Original language description
Sturgeons are the most endangered species group and their wild populations continue to decrease. In this study, we apply intracytoplasmic sperm injection (ICSI), an assisted reproductive technology, for the first time in endangered and critically endangered sturgeons. Using various egg-sperm species combinations we performed different ICSI experiments with immobilized pre- or non-activated spermatozoa, single or many, fresh or cryopreserved. Then we evaluated the fertilization success as well as the paternity of the resultant embryos and larvae. Surprisingly, all experimental groups exhibited embryonic development. Normal-shaped feeding larvae produced in all egg-sperm species-combination groups after ICSI using single fresh-stripped non-activated spermatozoa, in one group after ICSI using single fresh-stripped pre-activated spermatozoa, and in one group after ICSI using multiple fresh-stripped spermatozoa. ICSI with single cryopreserved non-activated spermatozoa produced neurula stage embryos. Molecular analysis showed genome integration of both egg- and sperm-donor species in most of the ICSI transplants. Overall, ICSI technology could be used as an assisted reproduction technique for producing sturgeons to rescue valuable paternal genomes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Veterinary Science
ISSN
2297-1769
e-ISSN
2297-1769
Volume of the periodical
9
Issue of the periodical within the volume
neuvedeno
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
nestrankovano
UT code for WoS article
000907793800001
EID of the result in the Scopus database
2-s2.0-85145654866