Hemocyte coagulation and phagocytic behavior in early stages of injury in crayfish (Arthropoda: Decapoda) affect their morphology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F23%3A43906219" target="_blank" >RIV/60076658:12520/23:43906219 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.dci.2022.104618" target="_blank" >https://doi.org/10.1016/j.dci.2022.104618</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dci.2022.104618" target="_blank" >10.1016/j.dci.2022.104618</a>
Alternative languages
Result language
angličtina
Original language name
Hemocyte coagulation and phagocytic behavior in early stages of injury in crayfish (Arthropoda: Decapoda) affect their morphology
Original language description
Crustacean hemocytes are important mediators of immune functions such as coagulation and phagocytosis. We employed an in situ approach to investigate the ultrastructural behavior of hemocytes during coagulation and phagocytosis in the early stages after injury caused by leg amputation, using transmission electron microscopy technique in marbled crayfish Procambarus virginalis. Hemocytes underwent drastic morphological changes during coagulation. The morphology of the cytoplasmic granules changed from electron-dense to electron-lucent forms in an expanding manner. The transformed granules containing amorphous electron-lucent material were observed to merge and discharge their contents into extracellular space for coagulation. We also observed that the contents of the nucleus participate in the process of coagulation. In addition, leg amputation induced extensive muscle degeneration and necrotic tissues were avidly taken up by the phagocytic hemocytes containing distinct phagosomes. Interestingly, we observed for the first time how the digested contents of phagocytized necrotic tissues are incorporated into granules and other cellular components that change the cell morphology by increasing the granularity of the hemocytes. Nevertheless, the degranulation of hemocytes during coagulation can also reduce their granularity. Given that morphological traits are important criteria for hemocyte classifi-cation, these morphological changes that occur during coagulation and phagocytosis must be taken into account.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Developmental and Comparative Immunology
ISSN
0145-305X
e-ISSN
1879-0089
Volume of the periodical
141
Issue of the periodical within the volume
neuvedeno
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
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UT code for WoS article
000924467100001
EID of the result in the Scopus database
2-s2.0-85146612460