High enrichment factors in chemical analysis of progestins and in bioassays: insights beyond trace levels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908163" target="_blank" >RIV/60076658:12520/24:43908163 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s11356-024-33714-y" target="_blank" >https://doi.org/10.1007/s11356-024-33714-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11356-024-33714-y" target="_blank" >10.1007/s11356-024-33714-y</a>
Alternative languages
Result language
angličtina
Original language name
High enrichment factors in chemical analysis of progestins and in bioassays: insights beyond trace levels
Original language description
Concerns are growing about adverse effects of progestins on biota, even at ultra-trace concentrations. The enrichment factor (EF) from extraction of analytes in environmental samples that is needed for sample pre-concentration can affect not only performance of the analytical method but also the matrix effect. Therefore, the present study aimed to assess the influence of high sample EF on performance of the high-performance liquid chromatography with atmospheric pressure chemical ionization and photoionization coupled with high-resolution mass spectrometry (HPLC-APCI/APPI-HRMS) method for analysis of progestins in waste water treatment plant (WWTP) effluents and surface waters and analysis of (anti-)progestogenic activities measured by (anti-)PR-CALUX bioassays. The results showed that HPLC-APCI/APPI-HRMS coupled with solid-phase extraction and a high EF (33,333 Lwater/Lextract) enabled the detection of more compounds compared to samples with lower sample EF (10,000 Lwater/Lextract). The matrix effect did not increase proportionally compared to lower EFs (10,000 and 16,666 Lwater/Lextract), and lower limits of quantification were achieved in WWTP effluents and surface waters. The results of bioassays have shown that relative EF of 25 Lwater/Lbioassay appears high enough to detect progestogenic activity in treated waste water. Our study is one of the first to provide insights into sample pre-concentration in analysis of progestins and progestogenicity in aquatic environments. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)
Result continuities
Project
<a href="/en/project/GA22-19136S" target="_blank" >GA22-19136S: Characterization of effects of substances with anti-progestogenic activity occurring in the aquatic environments on lower vertebrates</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Science and Pollution Research
ISSN
0944-1344
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
26
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
38500-38511
UT code for WoS article
999
EID of the result in the Scopus database
2-s2.0-85194528893