Structural and site-specific characterization of distinctiveNglycans with heavy fucosylation in human semen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43910164" target="_blank" >RIV/60076658:12520/25:43910164 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.carpta.2025.100941" target="_blank" >https://doi.org/10.1016/j.carpta.2025.100941</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carpta.2025.100941" target="_blank" >10.1016/j.carpta.2025.100941</a>
Alternative languages
Result language
angličtina
Original language name
Structural and site-specific characterization of distinctiveNglycans with heavy fucosylation in human semen
Original language description
Heavy fucosylation (fucose residues >= 6 per glycan) has been previously reported in human semen with unclear precise site-specific glycan structures. In current study, we characterized heavily fucosylated glycoproteins as a distinctive feature of human spermatozoa (HS) and seminal plasma (HSP), with a precise definition of glycan structural features at the glycosite-specific level. There were 49 unique heavily fucosylated intact glycopeptides (IGPs) at 15 N-glycosites from 12 glycoproteins identified in HS, and 188 unique heavily fucosylated IGPs at 58 N-glycosites from 37 glycoproteins in HSP. Among these heavily fucosylated glycoproteins, 10 were shared in HS and HSP, 2 were detected only in HS and 17 only in HSP. Almost all heavily fucosylated glycans were complex Nglycans with core fucosylation and Lewis antennary, among which CLU were glycosylated by ten and nine fucoses per glycan in HS and HSP, respectively. Moreover, these heavily fucosylated glycans varied from tri- to hexa-antennas. Notably, the N-glycan structures on shared heavily fucosylated glycoproteins were more complex in HSP than in HS. These heavily fucosylated glycoproteins identified in human semen represent a valuable and distinctive resource for glycopeptide studies, offering significant potential for advancing glycoproteomic methodologies and clinical research into male infertility.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Carbohydrate Polymer Technologies and Applications
ISSN
2666-8939
e-ISSN
2666-8939
Volume of the periodical
11
Issue of the periodical within the volume
neuvedeno
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
nestránkováno
UT code for WoS article
001539168300001
EID of the result in the Scopus database
2-s2.0-105010588191