The application of image cytometry for viability assessment of dual fluorescent - stained fish spermatozoa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F04%3A00005735" target="_blank" >RIV/60076658:12610/04:00005735 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The application of image cytometry for viability assessment of dual fluorescent - stained fish spermatozoa

Original language description

The viability of spermatozoa has been assessed using SYBR 14 staining for DNA of living cells and propidium iodide staining for DNA of degenerate cells. This dual staining was performed on four fish species (Siberian sturgeon, Acipenser baerii; common carp, Cyprinus carpio; tench, Tinca tinca and wets, Silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. Ten phase contrast and epifluorescent images were recorded per sample, corresponding images were overlaid, and the blended images were evaluated for live and dead spermatozoa, represented by green and red fluorescence signals. Live/dead proportions were assessed, after dual threshholding, by imaging software that counted absolute numbers of objects and computed their frequencies. All sperm heads were found to be labelled, emitting either green or red light. Mean numbers of spermatozoa per image were in the ranges 32-113, 61-105, 48-104 and 29-91 for Siberian s

Czech name

Použití obrazové cytometrie ke stanovení životaschopmosti duálně barvených rybích psermií

Czech description

Byla sledována životaschopnost spermatozoí při užití SYBR 14 barvení DNA živých buněk a barvení propidium jodidem u neživých buněk. Toto dvojí barvení bylo použito u čtyř druhů ryb (Acipenser baerii, Cyprinus carpio, Tinca tinca a Silurus glanis) a poměrmezi živými a mrtvými spermatozoi byl stanovován pomocí epifluorescenční mikroskopie a obrazové cytometrie.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Biology International

ISSN

1065-6995

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

28

Country of publishing house

BE - BELGIUM

Number of pages

5

Pages from-to

955-959

UT code for WoS article

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EID of the result in the Scopus database

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