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Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F08%3A00009358" target="_blank" >RIV/60076658:12640/08:00009358 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants

  • Original language description

    We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the las

  • Czech name

    Konvenční a kombinatoriální zobrazování fluorescence chlorofylu rostlin infikovaných tobamovirem

  • Czech description

    Porovnávali jsme efekt dvou kmenů jednoho viru (italský a španělský kmen tobamoviru PMMoV) v hostitelské rostlině Nicotiana benthamiana pomocí zobrazovací fluorimetrie. Infekce byla detekována změnou konvenčních parametrů fluorescence chlorofylu a/nebo za pomoci pokročilé statistické metody ? kombinatoriálního imagingu. Koncenční parametry detekovaly infekci v její střední fázi (tj. 16 dní po inokulaci), zatímco kombinatoriální imaging detekoval infekci již v její rané fázi - 4 dny po inokulaci v případě virulentnějšího kmene PMMoV-S a 6 dní po inokulaci pro méně virulentní kmen PMMoV-I.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Photosynthetica

  • ISSN

    0300-3604

  • e-ISSN

  • Volume of the periodical

    46

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000259257300016

  • EID of the result in the Scopus database