Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F06%3A00032051" target="_blank" >RIV/60077344:_____/06:00032051 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles
Original language description
For the first time, headspace solid-phase microextraction coupled with GC-MS analysis was used to study volatile compounds emitted by the tick Ixodes ricinus (L.). Variables such as the type of SPME fibre, equilibration time and extraction time have beenevaluated with the mixture of four selected standards, a so-called artificial tick (acetophenone, racemic 4-heptanolide, methyl 2-methoxybenzoate and methyl 3-chloro-4-methoxybenzoate). Optimized conditions were obtained by the use of PDMS 100 ?m fibreat 30 min equilibration and 15 min extraction time. The method proved to have a good linearity at a concentration range from 0.5 ng (1 ng for methyl 3-chloro-4-methoxybenzoate) to 25 ng. LODs for a compound ranged between 0.19 and 1 ng, RSD (%) ranged from 13.76 to 25.08. The determination of 1.99 ng of methyl 3-chloro-4-methoxybenzoate emitted by five engorged females proved the usefulness of the developed method to identify and quantify the volatile compounds emitted by ticks.
Czech name
Optimalizace metody SPME pro stanovení těkavých látek vylučovaných klíštětem Ixodes ricinus (L.)
Czech description
Metoda headspace SPME spojená s GC-MS analýzou byla poprvé použita pro studium těkavých látek vylučovaných klíštětem Ixodes ricinus (L.) Pro směs čtyř vybraných standardů, tzv. ?umělé klíště? (acetofenon, racemický 4-heptanolid, methyl 2-methoxybenzoát amethyl 3-chloro-4-methoxybenzoát) byly testovány tyto proměnné: typ SPME vlákna, doba ekvilibrace a doba extrakce. Jako optimální podmínky byly nalezeny: vlákno PDMS 100 ?m, 30 min ekvilibrace a 15 min extrakce. Metoda vykazovala dobrou linearitu v koncentračním rozmezí od 0,5 ng (1 ng for methyl 3-chloro-4-methoxybenzoát) do 25 ng. Limity detekce látek se pohybovaly v rozmezí 0,19 až 1 ng, RSD (%) od 13,76 do 25,08. Pomocí této vyvinuté metody bylo stanoveno 1,99 ng methyl 3-chloro-4-methoxybenzoátu emitovaného pěti nasátými samicemi. Byla tak dokázána schopnost metody identifikovat a kvantifikovat těkavé látky vylučované klíšťaty.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA206%2F04%2F0751" target="_blank" >GA206/04/0751: Chemical ecology of the tick Ixodes ricinus (L.) - study of female sexual pheromone</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
29
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
236-241
UT code for WoS article
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EID of the result in the Scopus database
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