Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00314557" target="_blank" >RIV/60077344:_____/08:00314557 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/08:00314557 RIV/60076658:12640/08:00009367
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes
Original language description
Gun4 is a porphyrin binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In this work we presented the functional analysis of Gun4 from the cyanobacterium Synechocystis 6803. Affinity co-purification of the FLAG-tagged Gun4 with the H subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4mutant strain that exhibited a decreased activity of magnesium chelatase and consequently appeared highly deficient in the cellular content of chlorophyll-binding proteins, especially photosystem II protein CP47
Czech name
Význam Gun4 proteinu u sinic pro metabolismus chlorofylu a skládání fotosyntetických komplexů
Czech description
Porfyrin-vazný protein Gun4 je znám jako aktivátor Mg-chelatázy - enzymu, katalizující jeden z kroků sysntézy chlorofylu. V této práci jsme analyzovali Gun4 protein sinice Synechocystis 6803. Gun4 byl ko-purifikován pomocí afinitní chromatografie spolu sH podjednotkou Mg-chelatázy, což potvrzuje asociaci Gun4 s tímto enzymem u sinic. Mutantní kmen s inaktivovaným genem pro Gun4 nebyl schopen fotoautotrofního růstu a vykazoval nízkou aktivitu Mg-chelatázy, následkem čehož měl tento kmen silně redukovanou hladinu chlorofyl-vazných proteinů, obvzláště proteinu CP47
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA500200713" target="_blank" >IAA500200713: New tools in molecular genetics of cyanobacteria - applications in exploration of the chlorophyll biosynthetic pathway</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
283
Issue of the periodical within the volume
38
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
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UT code for WoS article
000259200100005
EID of the result in the Scopus database
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