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Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00314557" target="_blank" >RIV/60077344:_____/08:00314557 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/08:00314557 RIV/60076658:12640/08:00009367

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes

  • Original language description

    Gun4 is a porphyrin binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In this work we presented the functional analysis of Gun4 from the cyanobacterium Synechocystis 6803. Affinity co-purification of the FLAG-tagged Gun4 with the H subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4mutant strain that exhibited a decreased activity of magnesium chelatase and consequently appeared highly deficient in the cellular content of chlorophyll-binding proteins, especially photosystem II protein CP47

  • Czech name

    Význam Gun4 proteinu u sinic pro metabolismus chlorofylu a skládání fotosyntetických komplexů

  • Czech description

    Porfyrin-vazný protein Gun4 je znám jako aktivátor Mg-chelatázy - enzymu, katalizující jeden z kroků sysntézy chlorofylu. V této práci jsme analyzovali Gun4 protein sinice Synechocystis 6803. Gun4 byl ko-purifikován pomocí afinitní chromatografie spolu sH podjednotkou Mg-chelatázy, což potvrzuje asociaci Gun4 s tímto enzymem u sinic. Mutantní kmen s inaktivovaným genem pro Gun4 nebyl schopen fotoautotrofního růstu a vykazoval nízkou aktivitu Mg-chelatázy, následkem čehož měl tento kmen silně redukovanou hladinu chlorofyl-vazných proteinů, obvzláště proteinu CP47

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/IAA500200713" target="_blank" >IAA500200713: New tools in molecular genetics of cyanobacteria - applications in exploration of the chlorophyll biosynthetic pathway</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biological Chemistry

  • ISSN

    0021-9258

  • e-ISSN

  • Volume of the periodical

    283

  • Issue of the periodical within the volume

    38

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

    000259200100005

  • EID of the result in the Scopus database