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Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00317620" target="_blank" >RIV/60077344:_____/08:00317620 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea

  • Original language description

    The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor.

  • Czech name

    Aktivita alkalických fosfatáz mořských bakterií studovaná pomocí substrátu ELF97: úspěchy a meze použitelnosti metody ve fosforem limitovaném Středozemním moři

  • Czech description

    Fluorogenní substrát ELF97 fosfát je hydrolyzován enzymem fosfatázou přičemž vzniká fluorescenční nerozpustný produkt ELF 97 alkohol (ELFA). Ve fosforem limitovaných bakteriálních kulturách bylo vysoké procento buněk značeno ELFA (26?100% v závislosti nakmenu). Naproti tomu ve vzorcích ze Středozemního moře to bylo jen 0,01%, přesto že byla prokázána limitace fosforem.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    DA - Hydrology and limnology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Aquatic Microbial Ecology

  • ISSN

    0948-3055

  • e-ISSN

  • Volume of the periodical

    52

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    7

  • Pages from-to

  • UT code for WoS article

    000259980500004

  • EID of the result in the Scopus database