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Efficiency of experimental infection of Ixodes ricinus ticks with Borrelia burgdorferi spirochetes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00320256" target="_blank" >RIV/60077344:_____/08:00320256 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/08:00009830

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Efficiency of experimental infection of Ixodes ricinus ticks with Borrelia burgdorferi spirochetes

  • Original language description

    Feeding of an ixodid tick on a Borrelia-infected vertebrate is the natural route of tick infection. To obtain a cohort of nymphs with high incidence of infection, the immersion of Ixodes ricinus larvae in the suspension of Borrelia burgdorferi sensu stricto spirochetes was used. Immersed larvae fed on C3H mice until full engorgement, molted to nymphs and by PCR with specific primers nine of ten nymphs were shown to be infected. Specific antibodies in sera of the mice, on which either immersion-infectedlarvae or infected nymphs had engorged, were not detected. However, nymphs molted from immersed larvae were able to infect na?ve mice, and all resulting ten adults were infected.

  • Czech name

    Účinnost experimentální infekce klíšťat Ixodes ricinus spirochetami Borrelia burgdorferi

  • Czech description

    Přirozenou cestou infekce klíšťat čeledi Ixodidae je sání klíštěte na obratlovci infikovaném spirochetami Lymeské boreliózy. Pro získání populace klíštěcích nymf s vysokou incidencí infekce byla použita metoda namáčení larev I. ricinus v suspenzi Borrelia burgdorferi sensu stricto. Po namáčení larvy sály na myších kmene C3H až do odpadnutí, metamorfovaly do stadia nymfy a byly vyšetřeny pomocí PCR se specifickými primery. Devět z deseti těchto nymf bylo infikováno spirochetami B. burgdorferi. V séru myší, na kterých sály namáčené larvy, ale také infikované nymfy, nebyly detekovány specifické protilátky. Nymfy pocházející z namáčených larev však byly schopny nakazit naivní myši, a všech 10 vyšetřených dospělců byly infikováno.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EC - Immunology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal of Medical Microbiology

  • ISSN

    1438-4221

  • e-ISSN

  • Volume of the periodical

    298

  • Issue of the periodical within the volume

    S1

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    36

  • Pages from-to

  • UT code for WoS article

    000258892100025

  • EID of the result in the Scopus database