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Photoperiodic induction of diapause requires regulated transcription of timeless in the larval brain of Chymomyza costata

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F08%3A00320520" target="_blank" >RIV/60077344:_____/08:00320520 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12410/08:00010621

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Photoperiodic induction of diapause requires regulated transcription of timeless in the larval brain of Chymomyza costata

  • Original language description

    Photoperiodic signal stimulates induction of larval diapause in Chymomyza costata. Larvae of NPD strain (npd-mutants) do not respond to photoperiod. Our previous results indicated that the locus npd could code for the timeless gene and its product mightrepresent a molecular link between circadian and photoperiodic clock systems. Here we present results of tim mRNA and TIM protein analyses in the larval brain. TIM protein was localized in 2 neurons of each brain hemisphere of the 4-d-old 3rd instar wild-type larvae. In a marked contrast, no TIM neurons were detected in the brain of 4-day-old 3rd instar npd-mutant larvae and the level of tim transcripts was approximately 10-fold lower in the NPD than in the wild-type strain. Daily changes in tim expression and TIM presence appeared to be under photoperiodic control in the wild-type larvae. Clear daily oscillations of tim transcription were observed during the development of 3rd instars under the short-day conditions.

  • Czech name

    Fotoperiodická indukce diapauzy v mozku larev Chymomyza costata vyžaduje regulovanou transkripci genu timeless

  • Czech description

    Fotoperiodický signál stimuluje indukci larvální diapauzy u Chymomyza costata. Larvy npd-mutantů na fotoperiodu neodpovídají.Předchozí výzkum ukázal, že npd lokus by mohl kódovat gen timeless a jeho produkt by tak mohl na molekulární úrovni reprezentovatspojení mezi cirkadiánním a fotoperiodickým hodinovým systémem. Protein TIM byl lokalizován ve dvou neuronech každé hemisféry u 4 dny staré larvy 3. instaru divokého kmene. Naproti tomu v mozku 4 dny staré larvy 3. instaru npd-mutantního kmene nebyl protein TIM detekován a množství mRNA transkriptu bylo přibližně 10x menší než u divokého kmene. Denní změny v expresi genu tim ukazují, že jsou fotoperiodicky řízeny. Jasné denní rytmy byly v průběhu vývoje 3. instaru pozorovány v podmínkách krátkého dne.Méně zřetelné oscilace byly zjištěny v podmínkách dlouhého dne, kde převažoval postupný vzrůst množství transkriptu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    ED - Physiology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biological Rhythms

  • ISSN

    0748-7304

  • e-ISSN

  • Volume of the periodical

    23

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000254471700003

  • EID of the result in the Scopus database