Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00331350" target="_blank" >RIV/60077344:_____/09:00331350 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio

Original language description

We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the nematode? (TTAGGC)n, arthropod? (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA206%2F06%2F1860" target="_blank" >GA206/06/1860: Molecular evolution of sex chromosomes in Lepidoptera</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome

ISSN

0831-2796

e-ISSN

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Volume of the periodical

52

Issue of the periodical within the volume

11

Country of publishing house

CA - CANADA

Number of pages

7

Pages from-to

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UT code for WoS article

000272206700001

EID of the result in the Scopus database

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