Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334941" target="_blank" >RIV/60077344:_____/09:00334941 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/09:00010592 RIV/60076658:12220/09:00010836

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

Original language description

A total of 995 faecal samples from slaughtered dairy cattle was examined microscopically for the presence of Cryptosporidium spp. Nested PCR amplifying the partial SSU rRNA and GP60 genes of Cryptosporidium was performed on all positive and 200 randomlyselected samples and the products were digested by SspI, VspI and MboII restriction enzymes. The highest prevalence of infection (18.2%) was in the animals in age group of 12-18 months. The sequence analyses of PCR-positive specimens identified 41 samples as C. andersoni (4.1%), 2 samples as C. bovis (0.2%), and 1 sample as C. parvum (0.1%). Cryptosporidium bovis was detected only in a group of cattle older than 18 months and C. parvum in heifer which was older than 14 months. Infection intensity was generally low. Sequence analysis of the GP60 gene showed that detected C. parvum belonged to the IIaA16G1R1 subtype. This is the first published report about subtyping of the Cryptosporidium GP60 gene from cattle in the Czech Republic.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/GP523%2F07%2FP117" target="_blank" >GP523/07/P117: Zoonotic potential of Cryptosporidium species infecting cattle and pigs</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

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Volume of the periodical

165

Issue of the periodical within the volume

1/2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

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UT code for WoS article

000271160200021

EID of the result in the Scopus database

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