Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00342948" target="_blank" >RIV/60077344:_____/10:00342948 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/10:00011920

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei

Original language description

We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC07032" target="_blank" >LC07032: Center for Functional Genetics</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology Research

ISSN

0932-0113

e-ISSN

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Volume of the periodical

106

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

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UT code for WoS article

000276234300030

EID of the result in the Scopus database

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