Biochemical properties of three plant nucleases with anticancer potential

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00357370" target="_blank" >RIV/60077344:_____/11:00357370 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/11:10303 RIV/60461373:22330/11:43870044

Result on the web

<a href="http://dx.doi.org/10.1016/j.plantsci.2010.10.006" target="_blank" >http://dx.doi.org/10.1016/j.plantsci.2010.10.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2010.10.006" target="_blank" >10.1016/j.plantsci.2010.10.006</a>

Result language

angličtina

Original language name

Biochemical properties of three plant nucleases with anticancer potential

Original language description

Biochemical and structural properties of three recombinant (R), highly homologous, plant bifunctional nucleases from tomato (R-TBN1), hop (R-HBN1) and Arabis brassica (R-ABN1) were determined. These nucleases cleave single- and double-stranded substrates, as well as both RNA and DNA with nearly the same efficiency. In addition, they are able to cleave several artificial substrates and highly stable viroid RNA. They also possess 3'-nucleotidase activity; therefore, they can be classified as nuclease I family members. Interestingly, poly(G) is resistant to cleavage and moreover it inhibits dsDNase, ssDNase and RNase activity of the studied nucleases. All three nucleases exhibit zinc-dependence and a strong stimulatory effect of Zn2+ for dsDNA cleavage. 3-D models, predicted on the basis of experimental structure of P1 nuclease, show nine amino acid residues responsible for interactions with zinc atoms, located in the same positions as in P1 nuclease.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F09%2F1214" target="_blank" >GA521/09/1214: Modification and in planta production of bifunctional nucleases and analysis of their anticancerogenic and biological activities</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

180

Issue of the periodical within the volume

2

Country of publishing house

IE - IRELAND

Number of pages

9

Pages from-to

343-351

UT code for WoS article

000286562200022

EID of the result in the Scopus database

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