Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00358401" target="_blank" >RIV/60077344:_____/11:00358401 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/11:43870554

Result on the web

<a href="http://dx.doi.org/10.1007/s10709-011-9546-4" target="_blank" >http://dx.doi.org/10.1007/s10709-011-9546-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10709-011-9546-4" target="_blank" >10.1007/s10709-011-9546-4</a>

Result language

angličtina

Original language name

Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

Original language description

We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg,whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetica

ISSN

0016-6707

e-ISSN

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Volume of the periodical

139

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

281-289

UT code for WoS article

000288514100001

EID of the result in the Scopus database

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